Radiation Oncology and Radiotherapy, Part A

1. DNA damage quantification by the COMET assay Mathias Weyland 2. Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells Ilio Vitale 3. Immunoblotting-based characterization of the DNA damage response Ilio Vitale 4. Assessment of lipid peroxidation in irradiated cells Boyi Gan, Chao Mao, Amber Horbath and Guang Lei 5. A simple method to assess clonogenic survival of irradiated cancer cells Maria Esperanza Rodriguez Ruiz 6. Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence Giulia Petroni 7. Cytofluorometric assessment of cell cycle progression in irradiated cells Giulia Petroni, Ai Sato, Aitziber Buque Martinez and Lorenzo Galluzzi 8. Assessment of transcription inhibition as a characteristic of immunogenic cell death Guido Kroemer, Oliver Kepp, Giulia Cerrato and Allan Sauvat 9. Assessment of eIF2a phosphorylation during immunogenic cell death Guido Kroemer, Oliver Kepp and Lucillia Bezu 10. Quantification of cytosolic DNA species by immunofluorescence and automated image analysis Lorenzo Galluzzi 11. Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death Jitka Fucikova 12. Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells Guido Kroemer, Oliver Kepp, Peng LIU and Liwei Zhao 13. Assessment of type I interferon responses as a feature of immunogenic cell death. Guido Kroemer, Oliver Kepp, Sabrina Forveille, Allan Sauvat and Liwei Zhao 14. Assessment of immunological memory formation in vivo Guido Kroemer, Oliver Kepp, Liwei Zhao and Peng LIU 15. Characterization of the SASP of cancer cells experiencing DNA damage Francis Rodier 16. ELISA-based quantification of type I IFN secretion by irradiated cancer cells Claire I. Vanpouille-Box 17. RT-PCR-assisted quantification of type I IFN responses by irradiated cancer cells Lorenzo Galluzzi 18. Methods to characterize the exosomal output of irradiated cancer cells Sheila Spada 19. Cytofluorometric assessment of acute cell death responses driven by radiation therapy Lorenzo Galluzzi

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• Biologia molecular
• Biologia Celular (citologia)
• Biologia do desenvolvimento

Annexin V; Apoptosis; ATAC-Seq; Automation; Beads; Biosensor; BrdU; Breast cancer; Cancer immunotherapy; Cell death; Cell stress; Cellular imaging; Cellular senescence; CGAS; Chemotherapy; Chromosomal instability; Click chemistry; CXCL10; Dactinomycin; DAMPs; DiOC6(3); DNA damage; Drug discovery; eIF2a; ELISA; Endoplasmic reticulum stress; Ferroptosis; FIJI; Gene editing; Histone mass spectrometry; Hypoxia; Image analysis; Immune checkpoint inhibitors; Immunofluorescence; Immunogenic cell death; Immunosuppression; Immunotherapy; In vivo experimentation; Innate immunity; Interferon type I; Lipid peroxidation; Local hypofractionated radiotherapy; Mass spectrometry; Micronuclei isolation; Mitochondrial DNA; Mitochondrial outer membrane permeabilization; mtDNA; MX1; PD-1; Pimonidazole; Plasma membrane permeabilization; Propidium iodide; Radiation; Radiation therapy; Reactive oxygen species; Regulated cell death; RNA synthesis; RUSH; SARRP; Sequential injections; STING1; Targeted anticancer agents; TS/A; Tumor; Tumor immunology; Type I interferon; Viral mimicry; Whole genome sequencing; Whole-genome bisulfite sequencing