Pseudokinases
-10%
portes grátis
Pseudokinases
Murphy, James; Jura, Natalia
Elsevier Science & Technology
05/2022
836
Dura
Inglês
9780323915410
15 a 20 dias
1450
Descrição não disponível.
1. Production and Purification of the PEAK pseudokinases for structural and functional studies
2. Structural biology and biophysical characterisation of Tribbles pseudokinases
3. Detecting endogenous TRIB protein expression and its downstream signalling
4. Analysis of human Tribbles 2 pseudokinase
5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
6. Characterization of pseudokinase ILK-mediated actin assembly
7. Biochemical examination of Titin pseudokinase
8. Approaches to study pseudokinase conformations
9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
10. Proximity ligation approaches to define the interactomes of pseudokinases
11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling
12. Strategies to study and stimulate bacterial pseudokinases
13. Dynamics of Kinases and Pseudokinases by HDX-MS
14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
15. Pseudoenzymology of Kinase Suppressor of Ras
16. Computational and evolutionary insights into pseudokinase mechanisms
17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
18. Pharmacological targeting of the pseudokinase HER3
19. Considerations and analysis of the unusual pseudokinase BUBR1
20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
21. Methods for discovering catalytic activities for pseudokinases
22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
24. Identification of small molecule binders for pseudokinases
25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
2. Structural biology and biophysical characterisation of Tribbles pseudokinases
3. Detecting endogenous TRIB protein expression and its downstream signalling
4. Analysis of human Tribbles 2 pseudokinase
5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
6. Characterization of pseudokinase ILK-mediated actin assembly
7. Biochemical examination of Titin pseudokinase
8. Approaches to study pseudokinase conformations
9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
10. Proximity ligation approaches to define the interactomes of pseudokinases
11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling
12. Strategies to study and stimulate bacterial pseudokinases
13. Dynamics of Kinases and Pseudokinases by HDX-MS
14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
15. Pseudoenzymology of Kinase Suppressor of Ras
16. Computational and evolutionary insights into pseudokinase mechanisms
17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
18. Pharmacological targeting of the pseudokinase HER3
19. Considerations and analysis of the unusual pseudokinase BUBR1
20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
21. Methods for discovering catalytic activities for pseudokinases
22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
24. Identification of small molecule binders for pseudokinases
25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Actin bundling; Activity-based probes; Acute myeloid leukemia; AKT; Allelic series; Allosteric; Allostery; Assay; ATP displacement; Atypical kinases; Ba/F3 cells; Bacterial protein expression; Baculovirus; Bioinformatics; Biolayer-interferometry (BLI); BUBR1; Bud32; Cancer; Cas9; Caulobacter crescentus; Cell adhesion; Cell death; Cell signaling; CETSA; cGMP; Complementation; Computational biology; Conformation; Coupled-enzyme assay; CRISPR; CRISPR-Cas9; Cryo-electron microscopy; Crystallization; Crystallography; Deucravacitinib; Differential scanning Fluorimetry; Dimerization; Drug discovery; DSF; EGFR; Evolutionary constraints; Flow cytometry; Fluorescence polarization; FSBA-cell lysate inactivation; Function; Galloway-Mowat syndrome; Gene editing; Genome editing; Growth factor; HER receptors; HER2; HER3; High-throughput screening; Histidine kinase; HTRF probe; Human epidermal growth factor receptors; Hydrogen-deuterium exchange; ILK; In silico protein stability calculation; Inhibitor; Inhibitor screening; Innate immunity; Integrin; Interfacial sites; Interleukin-1 receptor (IL1R); IRAK2; IRAK3; Isothermal titration calorimetry; ITC; Janus kinase family; KEOPS; KeywordPseudokinases; Kinase; Kinase activation; Kinase homology domain; Kinase inhibitors; Kinetochores; KSR; Leucine zipper; Ligand binding; Luminescence-based phosphotransfer activity assays; Medium-throughput cloning; MEK; Membrane proteins; Mitosis; MLKL; Molecular glues; Mouse models; Necroptosis; Oligomerization; Orthosteric site; PEAK pseudokinases; Phosphorylation; PP2A; Protein crystallization; Protein expression; Protein purification; Protein structure; Protein-drug interaction; Pseudoenzymes; Pseudokinase; Pseudokinases; RAF; Receptor guanylyl cyclase; Receptor tyrosine kinase (RTK); Receptor tyrosine kinases; Recombinant; Regulation; SBDD; Scaffolds; Screening; SCYL1; SCYL2; SCYL3; Sf21 cells; Signaling; Small molecule drugs; Structural biology; Structural mass spectrometry; Swarm plate assay; t6A; Target engagement; Thermal shift assay; Thermal stability; Toll-like receptor (TLR); TRIB1; TRIB2; Tribbles; tRNA modification; TSA; Two-component system; TYK2; TYK2-JH2; TYK2-JH2 PSEUDOKINASE DOMAIN BINDERS; Uncharacterized proteins; Webserver; Western blotting; X-ray crystallography; Zombie proteins
1. Production and Purification of the PEAK pseudokinases for structural and functional studies
2. Structural biology and biophysical characterisation of Tribbles pseudokinases
3. Detecting endogenous TRIB protein expression and its downstream signalling
4. Analysis of human Tribbles 2 pseudokinase
5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
6. Characterization of pseudokinase ILK-mediated actin assembly
7. Biochemical examination of Titin pseudokinase
8. Approaches to study pseudokinase conformations
9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
10. Proximity ligation approaches to define the interactomes of pseudokinases
11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling
12. Strategies to study and stimulate bacterial pseudokinases
13. Dynamics of Kinases and Pseudokinases by HDX-MS
14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
15. Pseudoenzymology of Kinase Suppressor of Ras
16. Computational and evolutionary insights into pseudokinase mechanisms
17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
18. Pharmacological targeting of the pseudokinase HER3
19. Considerations and analysis of the unusual pseudokinase BUBR1
20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
21. Methods for discovering catalytic activities for pseudokinases
22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
24. Identification of small molecule binders for pseudokinases
25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
2. Structural biology and biophysical characterisation of Tribbles pseudokinases
3. Detecting endogenous TRIB protein expression and its downstream signalling
4. Analysis of human Tribbles 2 pseudokinase
5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
6. Characterization of pseudokinase ILK-mediated actin assembly
7. Biochemical examination of Titin pseudokinase
8. Approaches to study pseudokinase conformations
9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
10. Proximity ligation approaches to define the interactomes of pseudokinases
11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling
12. Strategies to study and stimulate bacterial pseudokinases
13. Dynamics of Kinases and Pseudokinases by HDX-MS
14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
15. Pseudoenzymology of Kinase Suppressor of Ras
16. Computational and evolutionary insights into pseudokinase mechanisms
17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
18. Pharmacological targeting of the pseudokinase HER3
19. Considerations and analysis of the unusual pseudokinase BUBR1
20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
21. Methods for discovering catalytic activities for pseudokinases
22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
24. Identification of small molecule binders for pseudokinases
25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Actin bundling; Activity-based probes; Acute myeloid leukemia; AKT; Allelic series; Allosteric; Allostery; Assay; ATP displacement; Atypical kinases; Ba/F3 cells; Bacterial protein expression; Baculovirus; Bioinformatics; Biolayer-interferometry (BLI); BUBR1; Bud32; Cancer; Cas9; Caulobacter crescentus; Cell adhesion; Cell death; Cell signaling; CETSA; cGMP; Complementation; Computational biology; Conformation; Coupled-enzyme assay; CRISPR; CRISPR-Cas9; Cryo-electron microscopy; Crystallization; Crystallography; Deucravacitinib; Differential scanning Fluorimetry; Dimerization; Drug discovery; DSF; EGFR; Evolutionary constraints; Flow cytometry; Fluorescence polarization; FSBA-cell lysate inactivation; Function; Galloway-Mowat syndrome; Gene editing; Genome editing; Growth factor; HER receptors; HER2; HER3; High-throughput screening; Histidine kinase; HTRF probe; Human epidermal growth factor receptors; Hydrogen-deuterium exchange; ILK; In silico protein stability calculation; Inhibitor; Inhibitor screening; Innate immunity; Integrin; Interfacial sites; Interleukin-1 receptor (IL1R); IRAK2; IRAK3; Isothermal titration calorimetry; ITC; Janus kinase family; KEOPS; KeywordPseudokinases; Kinase; Kinase activation; Kinase homology domain; Kinase inhibitors; Kinetochores; KSR; Leucine zipper; Ligand binding; Luminescence-based phosphotransfer activity assays; Medium-throughput cloning; MEK; Membrane proteins; Mitosis; MLKL; Molecular glues; Mouse models; Necroptosis; Oligomerization; Orthosteric site; PEAK pseudokinases; Phosphorylation; PP2A; Protein crystallization; Protein expression; Protein purification; Protein structure; Protein-drug interaction; Pseudoenzymes; Pseudokinase; Pseudokinases; RAF; Receptor guanylyl cyclase; Receptor tyrosine kinase (RTK); Receptor tyrosine kinases; Recombinant; Regulation; SBDD; Scaffolds; Screening; SCYL1; SCYL2; SCYL3; Sf21 cells; Signaling; Small molecule drugs; Structural biology; Structural mass spectrometry; Swarm plate assay; t6A; Target engagement; Thermal shift assay; Thermal stability; Toll-like receptor (TLR); TRIB1; TRIB2; Tribbles; tRNA modification; TSA; Two-component system; TYK2; TYK2-JH2; TYK2-JH2 PSEUDOKINASE DOMAIN BINDERS; Uncharacterized proteins; Webserver; Western blotting; X-ray crystallography; Zombie proteins
